Histone Chaperone FACT Coordinates Nucleosome Interaction through Multiple Synergistic Binding Events
نویسندگان
چکیده
منابع مشابه
Histone chaperone FACT regulates homologous recombination by chromatin remodeling through interaction with RNF20.
The E3 ubiquitin ligase RNF20 regulates chromatin structure through ubiquitylation of histone H2B, so that early homologous recombination repair (HRR) proteins can access the DNA in eukaryotes during repair. However, it remains unresolved how RNF20 itself approaches the DNA in the presence of chromatin structure. Here, we identified the histone chaperone FACT as a key protein in the early steps...
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Histone H3 of nucleosomes positioned on active genes is trimethylated at Lys36 (H3K36me3) by the SETD2 (also termed KMT3A/SET2 or HYPB) methyltransferase. Previous studies in yeast indicated that H3K36me3 prevents spurious intragenic transcription initiation through recruitment of a histone deacetylase complex, a mechanism that is not conserved in mammals. Here, we report that downregulation of...
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Background: Rtt106 is a histone H3-H4 chaperone. Results: Rtt106 oligomerizes through its N terminus and binds (H3-H4)2 tetramers in vitro and in vivo. Conclusion: Rtt106 likely deposits (H3-H4)2 tetramers to promote nucleosome assembly. Significance:Determining the stoichiometry ofH3-H4molecules that associate with Rtt106will increase our understanding on how nucleosomes are formed during S ph...
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Changes in chromatin architecture induced by epigenetic mechanisms are essential for normal cellular processes such as gene expression, DNA repair, and cellular division. Compact chromatin presents a barrier to these processes and is highly regulated by epigenetic markers binding to components of the nucleosome. Histone modifications directly influence chromatin dynamics and facilitate recruitm...
متن کاملHistone Chaperone-Mediated Nucleosome Assembly Process
A huge amount of information is stored in genomic DNA and this stored information resides inside the nucleus with the aid of chromosomal condensation factors. It has been reported that the repeat nucleosome core particle (NCP) consists of 147-bp of DNA and two copies of H2A, H2B, H3 and H4. Regulation of chromosomal structure is important to many processes inside the cell. In vivo, a group of h...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2011
ISSN: 0021-9258
DOI: 10.1074/jbc.m111.301465